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InVivoMAb anti-mouse CD19
Clone Catalog # Category
1D3 BE0150 InVivoMab Antibodies
$300 - 3080

The 1D3 monoclonal antibody reacts with mouse CD19, a B cell-specific 95 kDa transmembrane glycoprotein of the immunoglobulin superfamily. CD19 contains two extracellular immunoglobulin-like domains and an extensive cytoplasmic tail. It functions as a positive regulator of B-cell receptor signaling in conjunction with CD21 and CD81. CD19 is highly expressed in most lymphomas and leukemias including some early B-cell malignancies that do not express CD20. For these reasons CD19 is quickly becoming an attractive alternative target for the immunotherapy of lymphoproliferative disorders. The 1D3 antibody has been reported to deplete B cells however, B cell depletion often requires treatment with a combination of 1D3, anti-mouse B220 (clone RA3.3A1/6.1), anti-mouse CD22 (clone Cy34.1), and anti-rat κ light chain (clone MAR 18.5) antibodies. Please see the provided references for protocol details.

Isotype

Rat IgG2a

Recomended Isotype Control(s) InVivoMAb rat IgG2a isotype control, anti-trinitrophenol
Recommended InVivoPure Dilution Buffer InVivoPure pH 7.0 Dilution Buffer
Immunogen

K562 cells expressing the extracellular domain of mouse CD19

Reported Applications
  • in vivo B cell depletion
  • in vivo CD19 neutralization
  • in vitro B cell negative selection
  • Flow cytometry
Endotoxin
  • <2EU/mg (<0.002EU/μg)
  • Determined by LAL gel clotting assay
Purity
  • >95%
  • Determined by SDS-PAGE
Formulation
  • PBS, pH 7.0
  • Contains no stabilizers or preservatives
Sterility

0.2 μM filtered

Production

Purified from tissue culture supernatant in an animal free facility

Purification

Protein G

Storage

Undiluted at 4°C in the dark