Frequently Asked Questions

  • You can place an order one of four ways:

    • 1. Email your purchase order to customerservice@bxcell.com. Your purchase order should include the items you are ordering and your shipping address and billing address.
    • 2. Fax your purchase order to 1-603-298-5226.
    • 3. Place your order on our website, bxcell.com using a credit card or PO number.
    • 4. Call us at 1-603-298-8564 and place your order with one of our customer service agents.
  • You can pay by check, credit card (VISA & Master Card), purchase order, or ACH and wire transfer (outside the USA). Our payment conditions are net 30.

  • Customers will be prompted to create a new account via submitting a new quote request or order. A new account can also be created by clicking on the account icon in the upper right corner of the page.

  • To reset your password click the “Lost Password” link at the bottom of the login window or click here.  Fill out the field on the password reset page with your Bio X Cell username or email address. Upon submission, an email will get sent to your account on file with a link to reset your password.

  • We do not offer free or trial sized samples for testing purposes.

  • First, please fill out and submit a cell line transfer sheet which can be found here. Then ship the cells frozen and packed with plenty of dry ice via FEDEX overnight delivery. Please do not ship on Thursdays. Make sure to put the “Dry Ice” label (black diamond) on the box.

  • Store your antibodies undiluted at 4°C in the dark. We do not recommend freezing antibodies (even when aliquoted) as this can result in a loss of activity caused by the freezing/thawing process. Additionally, diluting antibodies to working concentrations and storing at 4°C for more than a day should be avoided. Proteins, in general, are less susceptible to degradation when stored at higher concentrations. Under these storage conditions, your antibodies should remain active for up to one year and oftentimes longer (just make sure to keep the antibody sterile).

  • Always try to maintain the sterility of your antibodies to avoid contamination. Working with the antibodies in a biological safety cabinet and using sterile pipet tips, tubes, syringes, and buffers will greatly reduce the chances of contamination. Additionally, try to keep the antibodies cold until just prior to administration.

  • Bio X Cell’s products will remain stable for 1 year from the date of receipt if the antibodies are stored under the conditions outlined on our product data sheets.

  • Most of our antibodies are supplied as an aqueous solution in PBS containing 20mM NaH2PO4/KHPO4 and 135mM NaCl. However, we can supply antibodies in other buffers when requested. The pH will vary depending on the pI of the individual antibody. The buffer will not contain preservatives such as azide or protein stabilizers such as BSA.

  • The concentration of our products varies from lot to lot but is typically between 4 and 10 mg/ml. The exact concentration will be marked on the tube itself. If you need to know the exact concentration of a product before ordering, please contact technicalservice@bxcell.com.

  • We have not epitope mapped our antibodies at Bio X Cell. Searching the published literature would be the best way to determine if this information is available.

  • We recommend diluting our antibodies in the same buffer and at the same pH that they are supplied in. Our antibodies are supplied in PBS containing 20mM NaH2PO4/KHPO4 and 135mM NaCl. The pH will vary depending on the pI of the antibody and will be specified on the product data sheet and product webpage for each antibody.

    When diluting we recommend using cold PBS and maintaining sterility by working in a biological safety cabinet and using sterile pipet tips, tubes, syringes, and buffers.

    Diluting antibodies to working concentrations and storing at 4°C for more than a day should be avoided.

  • All of our products are suitable for in vivo use. They are supplied in PBS without preservatives such as azide or protein stabilizers such as BSA. The endotoxin levels are < 2EU/mg (InVivoMab) which qualifies them for most in vivo studies. To meet the most stringent requirements we also offer antibodies with lower endotoxin levels: <1EU/mg (InVivoPlus).

  • We do not have direct experimental data generated at Bio X Cell concerning the half-life of our antibodies in vivo. Antibody half-life can be highly variable and influenced by a variety of factors including species, isotype, antigen distribution, antigen concentration and many others. We recommend reading through the literature associated with the antibody you’re interested in to find published data in an experimental system similar to yours as a first step in estimating the half-life.

  • The optimal dose and frequency of administration for any given experiment and antibody can vary greatly based on many details including the experimental system (i.e. mouse strain, disease model, etc.), the duration of the experiment, the target tissue, the antigen concentration, and much more. For this reason, the optimal dose is best determined by reading through the product references associated with the antibody you’re interested in to find published data in an experimental system similar to yours. Using this as a starting point you can then optimize the dose and frequency of administration experimentally.

    Each of our products has an up-to-date reference list available on the products webpage.

  • Please see our InVivoPlus Difference page

  • Environmental conditions such as temperature variations, freezing/thawing, shaking during transport or long term storage might lead to protein aggregation. These aggregates are either settled in the conical tip of the vial or are floating freely in the suspension. These aggregates can be removed by gentle shaking at room temperature; they can also be removed by filtration or centrifugation without significant loss.