ReadyTag anti-OVA

CloneCatalog #Category
F2-3.58RT0267ReadyTag Antibodies
$95 - $1650

About ReadyTag anti-OVA

The F2-3.58 monoclonal antibody reacts with ovalbumin (OVA). OVA is a 45 kDa protein found in egg white that is commonly used in several different research areas. The protein is used to stimulate allergic inflammation in laboratory animals and is an established model allergen for airway hyper-responsiveness. OVA is also used in proteomics as a molecular weight marker.

ReadyTag anti-OVA Specifications

Isotype Mouse IgG1
Immunogen Phosphoprotein constituent of egg white
Formulation
  • PBS, pH 7
  • Contains no stabilizers or preservatives
Endotoxin
  • <2EU/mg (<0.002EU/μg)
  • Determined by LAL gel clotting assay
Purity
  • >95%
  • Determined by SDS-PAGE
Sterility 0.2 μM filtered
Production Purified from tissue culture supernatant in an animal free facility
Purification Protein G
RRID AB_2651130
Molecular Weight 150 kDa
Storage The antibody solution should be stored at the stock concentration at 4°C. Do not freeze.

Application References

ReadyTag anti-OVA (Clone: F2-3.58)

Qiao, S. W., et al. (2008). “Dependence of antibody-mediated presentation of antigen on FcRn.” Proc Natl Acad Sci U S A 105(27): 9337-9342. PubMed

The neonatal Fc receptor for IgG (FcRn) is a distant member of the MHC class I protein family. It binds IgG and albumin in a pH-dependent manner and protects these from catabolism by diverting them from a degradative fate in lysosomes. In addition, FcRn-mediated IgG transport across epithelial barriers is responsible for the transmission of IgG from mother to infant and can also enhance IgG-mediated antigen uptake across mucosal epithelia. We now show a previously undescribed role for FcRn in mediating the presentation of antigens by dendritic cells when antigens are present as a complex with antibody by uniquely directing multimeric immune complexes, but not monomeric IgG, to lysosomes.