ReadyTag anti-OVA

Clone Catalog # Category
F2-3.58 RT0267 ReadyTag Antibodies
$95 - $1650

About ReadyTag anti-OVA

The F2-3.58 monoclonal antibody reacts with ovalbumin (OVA). OVA is a 45 kDa protein found in egg white that is commonly used in several different research areas. The protein is used to stimulate allergic inflammation in laboratory animals and is an established model allergen for airway hyper-responsiveness. OVA is also used in proteomics as a molecular weight marker.

ReadyTag anti-OVA Specifications

Isotype

Mouse IgG1

Recommended InVivoPure Dilution Buffer InVivoPure pH 7.0 Dilution Buffer(IP0070)
Immunogen

Phosphoprotein constituent of egg white

Reported Applications:

ELISA

Endotoxin
  • <2EU/mg (<0.002EU/μg)
  • Determined by LAL gel clotting assay
Purity
  • >95%
  • Determined by SDS-PAGE
Formulation
  • PBS, pH 7
  • Contains no stabilizers or preservatives
Sterility

0.2 μM filtered

Production

Purified from tissue culture supernatant in an animal free facility

Purification

Protein A

Storage

The antibody solution should be stored at the stock concentration at 4°C. Do not freeze.

RRID

AB_2651130

Molecular Weight

150 kDa

Application References

ReadyTag anti-OVA (Clone: F2-3.58)

Qiao, S. W., et al. (2008). "Dependence of antibody-mediated presentation of antigen on FcRn." Proc Natl Acad Sci U S A 105(27): 9337-9342. PubMed The neonatal Fc receptor for IgG (FcRn) is a distant member of the MHC class I protein family. It binds IgG and albumin in a pH-dependent manner and protects these from catabolism by diverting them from a degradative fate in lysosomes. In addition, FcRn-mediated IgG transport across epithelial barriers is responsible for the transmission of IgG from mother to infant and can also enhance IgG-mediated antigen uptake across mucosal epithelia. We now show a previously undescribed role for FcRn in mediating the presentation of antigens by dendritic cells when antigens are present as a complex with antibody by uniquely directing multimeric immune complexes, but not monomeric IgG, to lysosomes.

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