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ReadyTag anti-GFP
Clone Catalog # Category
F56-6A1.2.3 RT0265 ReadyTag Antibodies
$300 - 4125

The F56-6A1.2.3 monoclonal antibody reacts with enhanced green fluorescent protein (GFP). GFP is a 27 kDa protein that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. In cell and molecular biology, GFP has become an invaluable tool. GFP is commonly used to visualize specific cell populations and as a reporter of gene expression and localization. YFP differs from GFP due to a mutation at T203Y; antibodies raised against full-length GFP should also detect YFP and other variants.

Isotype

Mouse IgG2b

Recommended InVivoPure Dilution Buffer InVivoPure pH 7.0 Dilution Buffer
Immunogen

Full length enhanced GFP

Reported Applications
  • Western blot
  • Immunofluorescence
Endotoxin
  • <2EU/mg (<0.002EU/μg)
  • Determined by LAL gel clotting assay
Purity
  • >95%
  • Determined by SDS-PAGE
Formulation
  • PBS, pH 7.0
  • Contains no stabilizers or preservatives
Sterility

0.2 μM filtered

Production

Purified from tissue culture supernatant in an animal free facility

Purification

Protein G

Storage

Undiluted at 4°C in the dark