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InVivoMAb anti-mouse CD3ε
Clone Catalog # Category
145-2C11 BE0001-1 InVivoMab Antibodies
$95 - $3080

The 145-2C11 monoclonal antibody reacts with mouse CD3ε, a 20 kDa transmembrane cell-surface protein that belongs to the immunoglobulin superfamily. CD3ε is one of five polypeptide chains that combine to form the TCR complex. CD3ε is expressed on T lymphocytes, NK-T cells, and to varying degrees on developing thymocytes. CD3 plays roles in TCR signaling, T lymphocyte activation, and antigen recognition. The 145-2C11 antibody has been shown to induce T lymphocyte activation, proliferation, and apoptosis in vitro via binding and stimulating the TCR. When used in vivo the antibody is reported to produce T cell activation, anergy, or death. Activation of resting T cells in vivo results in cytokine release and subsequent toxicity caused by Ab-mediated cross-linking of T cells and Fcγ receptor (FcR)-bearing cells. For this reason, the non-FcR-binding 145-2C11 f(ab')2 fragments are more commonly used for in vivo applications.  The 145-2C11 antibody has been reported to block the binding of the 17A2 antibody to CD3ε+ T lymphocytes.

Isotype

Armenian Hamster IgG1

Recomended Isotype Control(s) InVivoMAb polyclonal Armenian hamster IgG
Recommended InVivoPure Dilution Buffer InVivoPure pH 7.0 Dilution Buffer
Immunogen

Mouse BM10-37 cytotoxic T cells

Reported Applications
  • in vitro T cell stimulation/activation
  • Immunofluorescence
  • Flow cytometry
  • Western blot
  • in vivo T cell depletion (see description)
Endotoxin
  • <2EU/mg (<0.002EU/μg)
  • Determined by LAL gel clotting assay
Purity
  • >95%
  • Determined by SDS-PAGE
Formulation
  • PBS, pH 7.0
  • Contains no stabilizers or preservatives
Sterility

0.2 μM filtered

Production

Purified from tissue culture supernatant in an animal free facility

Purification

Protein A

Storage

Undiluted at 4°C in the dark

RRID

AB_1107634