InVivoMAb anti-mouse IL-27 p28

CloneCatalog #Category
MM27.7B1BE0326InVivoMab Antibodies
$95 - $3250

About InVivoMAb anti-mouse IL-27 p28

The MM27.7B1 monoclonal antibody reacts with the p28 subunit of mouse IL-27, a member of the IL-6/IL-12 cytokine family. IL-27 is a heterodimeric cytokine composed the EBI3 (Epstein-Barr virus induced gene 3) protein and the p28 subunit, also known as IL-30. Myeloid cells including macrophages, inflammatory monocytes, microglia, and dendritic cells are the dominant cellular sources of IL-27. These cells secrete IL-27 in response to TLR ligands and inflammatory cytokines. IL-27 p28 signals through IL-27 receptors (IL-27R) which are highly expressed by NK cells and activated T cells and to a lesser extent on B cells, naïve T cells, and others. IL-27 signaling has been shown to have both pro-inflammatory and anti-inflammatory effects. Signaling through IL-27R promotes Th1 polarization and IFNγ production but also suppresses the differentiation and proliferation of Th2 and Th17 cells and induces the expression of IL-10. The MM27.7B1 clone has been shown to neutralize the bioactivity of mouse IL-27 in vivo and in vitro and reported to also bind and inhibit human IL-27.

InVivoMAb anti-mouse IL-27 p28 Specifications

Isotype Mouse IgG2a, κ
Immunogen Mouse IL-27
Reported Applications
  • in vivo IL-27 p28 neutralization
  • in vitro IL-27 p28 neutralization
  • Flow cytometry
Formulation
  • PBS, pH 7.0
  • Contains no stabilizers or preservatives
Endotoxin
  • <2EU/mg (<0.002EU/μg)
  • Determined by LAL gel clotting assay
Purity
  • >95%
  • Determined by SDS-PAGE
Sterility 0.2 μM filtered
Production Purified from tissue culture supernatant in an animal free facility
Purification Protein A
RRID AB_2819053
Molecular Weight 150 kDa
Storage The antibody solution should be stored at the stock concentration at 4°C. Do not freeze.