InVivoMAb anti-mouse CD45.2

Clone Catalog # Category
104.2 BE0300 InVivoMab Antibodies
$95 - $3080
SDS Sheet

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About InVivoMAb anti-mouse CD45.2

The 104.2 monoclonal antibody reacts with mouse CD45.2 a 180-240 kDa member of the protein tyrosine phosphatase family. CD45.2 is an alloantigen of CD45 and is only expressed by certain mouse strains including C57BL/6, CBA, 129, A, AKR, C58, DBA/1, DBA/2, BALB/c, and C3H/He. The 104.2 monoclonal antibody does not react with leukocytes of CD45.1-expressing mouse strains including DA, SJL/J, RIII, and STS/A. CD45.2 is expressed on all hematopoietic cells except mature erythrocytes and platelets and is thought to be involved in TCR and BCR signal transduction. The 104.2 antibody has been shown to block B cell responses in vitro and reduce serum autoantibody concentration in vivo in SLE-prone mice.

InVivoMAb anti-mouse CD45.2 Specifications

Isotype

Mouse IgG2a, κ

Recomended Isotype Control(s) InVivoMAb mouse IgG2a isotype control, unknown specificity(BE0085)
Recommended InVivoPure Dilution Buffer InVivoPure pH 7.0 Dilution Buffer(IP0070)
Immunogen

B10.S mouse thymocytes and splenocytes

Reported Applications
  • Flow cytometry
  • in vivo CD45.2 blockade
  • in vitro CD45.2 blockade
  • Immunohistochemistry (frozen)
Endotoxin
  • <2EU/mg (<0.002EU/μg)
  • Determined by LAL gel clotting assay
Purity
  • >95%
  • Determined by SDS-PAGE
Formulation
  • PBS, pH 7.0
  • Contains no stabilizers or preservatives
Sterility

0.2 μm filtration

Production

Purified from tissue culture supernatant in an animal free facility

Purification

Protein G

Storage

The antibody solution should be stored undiluted at 4°C, and protected from prolonged exposure to light. Do not freeze.

RRID

AB_2715458