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InVivoMAb anti-human/monkey CD20
Clone Catalog # Category
2H7 BE0276 InVivoMab Antibodies
$300 - 3080

The 2H7 monoclonal antibody reacts with human and primate CD20. CD20 is a B cell-specific 33-37 kDa transmembrane protein which is also known as B-lymphocyte antigen, B1, and Bp35. CD20 plays roles in intracellular calcium regulation and B cell activation and is critical for an optimal B cell immune response against T-independent antigens. CD20 is first expressed after the induction of CD19 together with IgM during the pre-B to immature B cell transition in the bone marrow. It’s expression then increases during maturation with almost all mature B cells expressing some level of CD20. However, CD20 is not expressed by plasma blasts or plasma cells. CD20 is expressed by most B cell neoplasms, and is useful in diagnosing B cell lymphomas and leukemia. Many anti-CD20 monoclonal antibodies are currently being used to successfully treat leukemia, lymphomas, and various autoimmune diseases. The 2H7 monoclonal antibody has been shown to bind to an epitope in the large extracellular loop of human CD20.

Isotype

Mouse IgG2b

Recomended Isotype Control(s) InVivoMAb mouse IgG2b isotype control, unknown specificity
Recommended InVivoPure Dilution Buffer InVivoPure pH 7.0 Dilution Buffer
Immunogen

Human tonsillar B cells

Reported Applications
  • in vivo B cell depletion in hCD20 Tg mice
  • Immunohistochemistry (frozen)
  • Immunoprecipitation
  • Flow cytometry
Endotoxin
  • <2EU/mg (<0.002EU/μg)
  • Determined by LAL gel clotting assay
Purity
  • >95%
  • Determined by SDS-PAGE
Formulation
  • PBS, pH 7.0
  • Contains no stabilizers or preservatives
Sterility

0.2 μM filtered

Production

Purified from tissue culture supernatant in an animal free facility

Purification

Protein G

Storage

Undiluted at 4°C in the dark