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InVivoMAb anti-human CD32 (FcγRIIA)
Clone Catalog # Category
IV.3 BE0224 InVivoMab Antibodies
$300 - 3080

The IV.3 monoclonal antibody reacts with human CD32 also known as FcγRII and FCRII, a 40 kDa polymorphic transmembrane glycoprotein and an Ig superfamily member. CD32 is expressed on monocytes/macrophages, granulocytes, platelets and B cells. CD32 enables interaction between Fc γ RII-expressing cells and opsonized antigen or IgG-containing immune complexes. This allows CD32 to function in the activation or inhibition of immune responses including degranulation, phagocytosis, ADCC, cytokine release, and B cell proliferation. The IV.3 antibody has been shown to block the biological effects of CD32 in vitro. Additionally, IV.3 f(ab')2 fragments have been used to block CD32 in vivo in transgenic mice expressing human CD32.

Isotype

Mouse IgG2b

Recomended Isotype Control(s) InVivoMAb mouse IgG2b isotype control, unknown specificity
Recommended InVivoPure Dilution Buffer InVivoPure pH 7.0 Dilution Buffer
Immunogen

Human K562 leukemia cell line

Reported Applications
  • in vivo FcγRIIA blockade in humanized mice
  • in vitro FcγRIIA blockade
  • ELISA
  • Flow cytometry
Endotoxin
  • <2EU/mg (<0.002EU/μg)
  • Determined by LAL gel clotting assay
Purity
  • >95%
  • Determined by SDS-PAGE
Formulation
  • PBS, pH 7.0
  • Contains no stabilizers or preservatives
Sterility

0.2 μM filtered

Production

Purified from tissue culture supernatant in an animal free facility

Purification

Protein G

Storage

Undiluted at 4°C in the dark