Don’t have time to grow and purify your antibody from a hybridoma?
We can help!
- 25 years of experience
- Fast turnaround time
- <2EU/mg endotoxin
- Ultra-high purity
- Customizable with à la carte services
- Regular updates from a project manager
Antibody Production Services
At Bio X Cell, we know that growing hybridoma cells and purifying antibodies in large quantities with low endotoxin levels can be technically challenging and time-consuming. Enlist our experienced team to do this for you! Bio X Cell’s optimized fermentation process produces more antibody in less time than traditional methods. A technical project manager will guide you through the process, providing you with recommendations and updates along the way.
Our services include:
- Antibody Production from Hybridoma Cells, Milligrams to Grams
- Cell Freezing and Storage
- Mycoplasma Testing
- Isotyping
- Murine Pathogen Screening
- Isotype Control Antibodies
- InVivoPure Dilution Buffers
We know that research moves at a fast and precise pace, and we aim to partner with you to achieve your goals. Our platform is customizable, allowing you to collaborate with our team to select à la carte services that improve the rigor of your experimental design. If you do not see a service listed, e-mail us at customerservice@bxcell.com to provide more details about your project.
Project Workflow
| Phase | Description | Included | Additional Fee |
|---|---|---|---|
| 1. Pilot | Instructions for preparing and shipping cells. | ✔ | |
| Assessment of cell line productivity: >30mg of antibody/Liter is required or Bio X Cell cannot proceed to scale up and a pilot fee will be charged ($800 Academic, $1,000 Commercial). | ✔ | ||
| Mycoplasma testing service. | ✔ | ||
| Isotyping of cell culture supernatant. | ✔ | ||
| 2. Fermentation | Cell culturing and monitoring 7 days/week. | ✔ | |
| Freezing of five vials of cells. | ✔ | ||
| Storage of cells at Bio X Cell for rapid resupply. | ✔ | ||
| Expansion of cells using optimized fermentation process. | ✔ | ||
| Concentration of cell supernatant. | ✔ | ||
| 3. Purification | Purification of supernatant on Protein A or G. | ✔ | |
| Buffer exchange to PBS that is preservative and stabilizer free. | ✔ | ||
| Sterile filtering of final product. | ✔ | ||
| Testing to confirm <2EU/mg endotoxin. <1EU/mg available for an extra charge. |
✔ | ||
| 4. Vialing and QC | Sterile vialing of finished product. Custom aliquoting available for an extra charge. |
✔ | |
| Internal antibody quality assessment. | ✔ | ||
| Certificate of Analysis | ✔ | ||
| Murine pathogen screening service. | ✔ | ||
| 5. Shipping | Shipping on cold packs in environmentally friendly packaging. | ✔ |
Pricing
| Size | 50 mg | 100 mg | 200 mg | 500 mg * |
|---|---|---|---|---|
| Academic | $1,920 | $3,070 | $4,910 | $9,900 |
| Commercial | $2,950 | $4,720 | $7,550 | $15,200 |
* For pricing greater than 500 mg, please e-mail customerservice@bxcell.com.