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InVivoMAb anti-mouse/human T-bet
Clone Catalog # Category
4B10 BE0100 InVivoMab Antibodies
$300 - 3080

The 4B10 monoclonal antibody reacts with mouse and human T-bet also known as T-box transcription factor. T-bet is expressed by many hematopoietic cells including stem cells, NK cells, B lymphocytes, and T lymphocytes. Studies in T-bet deficient mice have revealed that T-bet is a Th1 cell-specific transcription factor that controls the expression of the hallmark Th1 cytokine, IFNγ. T-bet expression is optimally observed after IL-12 stimulation and can be suppressed by addition of the Th2 cytokine IL-4 or neutralization of IL-12.

Isotype

Mouse IgG1

Recomended Isotype Control(s) InVivoMAb mouse IgG1 isotype control, unknown specificity
Recommended InVivoPure Dilution Buffer InVivoPure pH 7.0 Dilution Buffer
Immunogen

Recombinant mouse T-bet

Reported Applications
  • Western blot
  • Immunoprecipitation
  • Immunohistochemistry (paraffin)
  • Flow cytometry
Endotoxin
  • <2EU/mg (<0.002EU/μg)
  • Determined by LAL gel clotting assay
Purity
  • >95%
  • Determined by SDS-PAGE
Formulation
  • PBS, pH 7.0
  • Contains no stabilizers or preservatives
Sterility

0.2 μM filtered

Production

Purified from tissue culture supernatant in an animal free facility

Purification

Protein G

Storage

Undiluted at 4°C in the dark