RecombiMAb human IgG4 S228P L235E P329G (SPLEPG) isotype control, anti-hen egg lysozyme

CloneCatalog #Category
N/A-CP148 CP148Isotype Controls
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Technical Data Sheet
SDS Sheet

About RecombiMAb human IgG4 S228P L235E P329G (SPLEPG) isotype control, anti-hen egg lysozyme

This human IgG4 isotype control antibody reacts with hen egg lysozyme and has low or no specific binding to any human sample. The S228P substitution is included to prevent Fab-arm exchange. The P329G substitution combined with S228P and L235E substitutions has been shown to decrease effector functions by disrupting the Fc/Fcγ receptor interface.

RecombiMAb human IgG4 S228P L235E P329G (SPLEPG) isotype control, anti-hen egg lysozyme Specifications

Isotype Human IgG4
Recommended Dilution Buffer InVivoPure™ pH 7.0 Dilution Buffer
Mutations S228P, L235E, P329G (SPLEPG)
Immunogen Hen egg lysozyme (HEL)
Formulation
  • PBS, pH 7.0
  • Contains no stabilizers or preservatives
Endotoxin
  • <1EU/mg (<0.001EU/μg)
  • Determined by LAL gel clotting assay
Aggregation
  • <5%
  • Determined by DLS
Purity
  • >95%
  • Determined by SDS-PAGE
Sterility 0.2 μM filtered
Production Purified from CHO cell supernatant
Purification Protein A
Molecular Weight 150 kDa
Murine Pathogen Test Results
  • Mouse Norovirus: Negative
  • Mouse Parvovirus: Negative
  • Mouse Minute Virus: Negative
  • Mouse Hepatitis Virus: Negative
  • Reovirus Screen: Negative
  • Lymphocytic Choriomeningitis virus: Negative
  • Lactate Dehydrogenase-Elevating Virus: Negative
  • Mouse Rotavirus: Negative
  • Theiler’s Murine Encephalomyelitis: Negative
  • Ectromelia/Mousepox Virus: Negative
  • Hantavirus: Negative
  • Polyoma Virus: Negative
  • Mouse Adenovirus: Negative
  • Sendai Virus: Negative
  • Mycoplasma Pulmonis: Negative
  • Pneumonia Virus of Mice: Negative
  • Mouse Cytomegalovirus: Negative
  • K Virus: Negative
Storage The antibody solution should be stored at the stock concentration at 4°C. Do not freeze.