InVivoMAb anti-mouse MHC Class II (I-A/I-E)

Clone Catalog # Category
M5/114 BE0108 InVivoMab Antibodies
$95 - $3080
SDS Sheet

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About InVivoMAb anti-mouse MHC Class II (I-A/I-E)

The M5/114 monoclonal antibody reacts with mouse MHC Class II haplotypes I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek. The antibody does not react with I-Af, I-Ak, or I-As haplotypes. The M5/114 antibody is reported to inhibit I-A-restricted T cell responses.

InVivoMAb anti-mouse MHC Class II (I-A/I-E) Specifications

Isotype

Rat IgG2b

Recomended Isotype Control(s) InVivoMAb rat IgG2b isotype control, anti-keyhole limpet hemocyanin(BE0090)
Recommended InVivoPure Dilution Buffer InVivoPure pH 7.0 Dilution Buffer(IP0070)
Immunogen

Activated C57BL/6 mouse spleen cells

Reported Applications
  • in vivo MHC II blockade
  • Functional assays
  • Immunofluorescence
  • Western blot
  • Immunoprecipitation
  • Flow cytometry
Endotoxin
  • <2EU/mg (<0.002EU/μg)
  • Determined by LAL gel clotting assay
Purity
  • >95%
  • Determined by SDS-PAGE
Formulation
  • PBS, pH 7.0
  • Contains no stabilizers or preservatives
Sterility

0.2 μM filtered

Production

Purified from tissue culture supernatant in an animal free facility

Purification

Protein G

Storage

The antibody solution should be stored undiluted at 4°C, and protected from prolonged exposure to light. Do not freeze.

RRID

AB_10949298