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InVivoMAb anti-mouse MHC Class I (H-2Kb) bound to SIINFEKL peptide (OVA residues 257-264)
Clone Catalog # Category
25-D1.16 BE0207 InVivoMab Antibodies
$95 - $3080

The 25-D1.16 monoclonal antibody reacts with mouse MHC class I H-2Kb bound to the ovalbumin-derived peptide with sequence SIINFEKL. This antibody does not react with unbound MHC class I H-2Kb or MHC class I H-2Kb bound to an irrelevant peptide. The 25-D1.16 antibody is often used to track the quantity and localization of antigen-presenting cells bearing these specific molecules in vivo.

Isotype

Mouse IgG1, κ

Recomended Isotype Control(s) InVivoMAb mouse IgG1 isotype control, unknown specificity
Recommended InVivoPure Dilution Buffer InVivoPure pH 7.0 Dilution Buffer
Immunogen

SIINFEKL pulsed RMA-S cells

Reported Applications
  • Functional assays
  • Flow cytometry
Endotoxin
  • <2EU/mg (<0.002EU/μg)
  • Determined by LAL gel clotting assay
Purity
  • >95%
  • Determined by SDS-PAGE
Formulation
  • PBS, pH 7.0
  • Contains no stabilizers or preservatives
Sterility

0.2 μM filtered

Production

Purified from tissue culture supernatant in an animal free facility

Purification

Protein G

Storage

Undiluted at 4°C in the dark

RRID

AB_10950697