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InVivoMAb anti-mouse TNF╬▒
Clone Catalog # Category
XT3.11 BE0058 InVivoMab Antibodies
$300 - 3080

The XT3.11 monoclonal antibody reacts with mouse TNF╬▒ (tumor necrosis factor-alpha) a multifunctional proinflammatory cytokine. TNF╬▒ exists as a soluble 17 kDa monomer, which forms homotrimers in circulation or as a 26 kDa membrane-bound form. TNF╬▒ belongs to the TNF superfamily of cytokines and signals through its two receptors, TNFR1 and TNFR2 which can be activated by both the soluble trimeric and membrane-bound and forms of TNF╬▒. TNF╬▒ is primarily produced by macrophages in response to foreign antigens such as bacteria (lipopolysaccharides), viruses, and parasites as well as mitogens and other cytokines but can also be expressed by monocytes, neutrophils, NK cells, CD4 T cells and some specialized dendritic cells. TNF╬▒ is known to play key roles in a wide spectrum of biological processes including immunoregulation, cell proliferation, differentiation, apoptosis, antitumor activity, inflammation, anorexia, cachexia, septic shock, hematopoiesis, and viral replication. TNF╬▒ dysregulation has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Mouse and human TNF╬▒ share 79% amino acid sequence identity however, mouse TNF╬▒ is glycosylated while human TNF╬▒ is not. TNF╬▒ knockout animals display defects in response to bacterial infection, characterized by defects in forming organized follicular dendritic cell networks and germinal centers with a lack of primary B cell follicles.

Isotype

Rat IgG1

Recomended Isotype Control(s) InVivoMAb rat IgG1 Isotype control, anti-trinitrophenol
InVivoMAb rat IgG1 isotype control, anti-horseradish peroxidase
Recommended InVivoPure Dilution Buffer InVivoPure pH 8.0 Dilution Buffer
Immunogen

Recombinant mouse TNF╬▒

Reported Applications
  • in vivo TNF╬▒ neutralization
  • in vitro TNF╬▒ neutralization
  • Western blot
Endotoxin
  • <2EU/mg (<0.002EU/╬╝g)
  • Determined by LAL gel clotting assay
Purity
  • >95%
  • Determined by SDS-PAGE
Formulation
  • PBS, pH 8.0
  • Contains no stabilizers or preservatives
Sterility

0.2 ╬╝M filtered

Production

Purified from tissue culture supernatant in an animal free facility

Purification

Protein G

Storage

Undiluted at 4┬░C in the dark