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InVivoMAb anti-mouse CD8β (Lyt 3.2)
Clone Catalog # Category
53-5.8 BE0223 InVivoMab Antibodies
$300 - 3080

The 53-5.8 monoclonal antibody reacts with mouse CD8β also known as Lyt 3.2. The CD8 antigen is a transmembrane glycoprotein that acts as a co-receptor for the T cell receptor (TCR). Like the TCR, CD8 binds to class I MHC molecules displayed by antigen presenting cells (APC). CD8 is primarily expressed on the surface of cytotoxic T cells, but can also be found on thymocytes, natural killer cells, and some dendritic cell subsets. CD8 most commonly exists as a heterodimer composed of one CD8α and one CD8β chain however, it can also exist as a homodimer composed of two CD8α chains. Both the CD8α and CD8β chains share significant homology to immunoglobulin variable light chains. The molecular weight of each CD8 chain is approximately 34 kDa. The 53-5.8 antibody has been shown to deplete CD8+ T cells completely but not deplete CD8+ CD11c+ dendritic cells when used in vivo.

Isotype

Rat IgG1

Recomended Isotype Control(s) InVivoMAb rat IgG1 Isotype control, anti-trinitrophenol
InVivoMAb rat IgG1 isotype control, anti-horseradish peroxidase
Recommended InVivoPure Dilution Buffer InVivoPure pH 7.0 Dilution Buffer
Immunogen

Mouse thymus or spleen

Reported Applications
  • in vivo CD8+ T cell depletion
  • in vitro CD8 blockade
  • Immunofluorescence
Endotoxin
  • <2EU/mg (<0.002EU/μg)
  • Determined by LAL gel clotting assay
Purity
  • >95%
  • Determined by SDS-PAGE
Formulation
  • PBS, pH 7.0
  • Contains no stabilizers or preservatives
Sterility

0.2 μM filtered

Production

Purified from tissue culture supernatant in an animal free facility

Purification

Protein G

Storage

Undiluted at 4°C in the dark