InVivoMAb anti-mouse MHC Class II (βchain)

Clone Catalog # Category
KL277 BE0140 InVivoMab Antibodies
$95 - $3250

About InVivoMAb anti-mouse MHC Class II (βchain)

The KL277 monoclonal antibody reacts with mouse MHC Class II haplotypes I-Ab, I-Ad, I-Ap, I-Aq, I-Abu, and I-Abw. The antibody does not react with I-Aa, I-Ak, I-Af, I-Aj, I-As, or I-Atl haplotypes.

InVivoMAb anti-mouse MHC Class II (βchain) Specifications

Isotype

Hamster/Mouse IgG

Recommended InVivoPure Dilution Buffer InVivoPure pH 7.0 Dilution Buffer(IP0070)
Immunogen

Synthetic peptide corresponding to residues 146-177 of the mouse Ab/beta protein

Reported Applications

Western blot

Endotoxin
  • <2EU/mg (<0.002EU/μg)
  • Determined by LAL gel clotting assay
Purity
  • >95%
  • Determined by SDS-PAGE
Formulation
  • PBS, pH 7.0
  • Contains no stabilizers or preservatives
Sterility

0.2 μM filtered

Production

Purified from tissue culture supernatant in an animal free facility

Purification

Protein G

Storage

The antibody solution should be stored at the stock concentration at 4°C. Do not freeze.

RRID

AB_10951148

Molecular Weight

150 kDa

Application References

InVivoMAb anti-mouse MHC Class II (β chain) (Clone: KL277)

  Sofron, A., et al. (2015). "High-resolution analysis of the murine MHC class II immunopeptidome." Eur J Immunol. doi: 10.1002/eji.201545930. PubMed

The reliable identification of peptides bound to MHC class II is fundamental for the study of the host immune response against pathogens and the pathogenesis of autoimmune conditions. Here we describe an improved methodology combining immuno-affinity enrichment of MHC class II complexes, optimized elution conditions and quadrupole Orbitrap mass spectrometry-based characterization of the immunopeptidome. The methodology allowed the identification of over 1000 peptides with 1% false discovery rate from 108 murine A20 lymphoma cells. The study revealed the I-Ad specific motif in high resolution after multi-sequence alignment. The methodology was generally applied to the purification of MHC class II from cell lines and murine spleens. We identified 2963 peptides from BALB/c and 2712 from C57BL/6 mouse spleens. The identification of peptides that bound to MHC class II in vitro and in vivo will facilitate the characterization of T-cell specificities, as well as the development of biotherapeutics and vaccines. This article is protected by copyright. All rights reserved.

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