InVivoMAb anti-mouse CD73

Clone Catalog # Category
TY/23 BE0209 InVivoMab Antibodies
$95 - $3080
SDS Sheet

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About InVivoMAb anti-mouse CD73

The TY/23 monoclonal antibody reacts with mouse CD73 also known as ecto-5'-nucleotidase (5'-NT), a 69 kDa GPI-anchored cell-surface protein. In mice, expression of CD73 is restricted to CD11b+ myeloid cells in the bone marrow and T cells in the spleen. CD73 plays a strategic role in calibrating the duration, magnitude, and chemical nature of purinergic signals delivered to immune cells through the conversion of AMP to adenosine. This drives a shift from an ATP-driven proinflammatory environment to an anti-inflammatory milieu induced by adenosine. CD73 has been shown to be important for the immunosuppressive activity of regulatory T cells. The TY/23 antibody has been reported to inhibit the enzymatic activity of CD73.

InVivoMAb anti-mouse CD73 Specifications

Isotype

Rat IgG2a, κ

Recommended Isotype Control(s) InVivoMAb rat IgG2a isotype control, anti-trinitrophenol(BE0089)
Recommended InVivoPure Dilution Buffer InVivoPure pH 7.0 Dilution Buffer(IP0070)
Immunogen

BALB/c mouse spleen cells and CHO cells transfected with the mouse CD73 gene

Reported Applications

in vivo CD73 blockade

Endotoxin
  • <2EU/mg (<0.002EU/μg)
  • Determined by LAL gel clotting assay
Purity
  • >95%
  • Determined by SDS-PAGE
Formulation
  • PBS, pH 7.0
  • Contains no stabilizers or preservatives
Sterility

0.2 μM filtered

Production

Purified from tissue culture supernatant in an animal free facility

Purification

Protein G

Storage

The antibody solution should be stored undiluted at 4°C, and protected from prolonged exposure to light. Do not freeze.

RRID

AB_10950310