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InVivoMAb anti-mouse CD54 (ICAM-1)
Clone Catalog # Category
YN1/1.7.4 BE0020-1 InVivoMab Antibodies
$300 - 3080

The YN1/1.7.4 monoclonal antibody reacts with mouse CD54 also known as Intercellular Adhesion Molecule-1 (ICAM-1). CD54 is a 90 kDa single-chain transmembrane glycoprotein and a member of the Ig superfamily. CD54 is typically expressed on non-hematopoietic cells such as endothelial cells, thymic epithelial cells, and fibroblasts but can also be expressed on macrophages, T-lymphoblasts, germinal center B cells and dendritic cells. CD54 is a ligand for LFA-1, a receptor found on leukocytes. When activated, leukocytes bind to endothelial cells via CD54/LFA-1 to facilitate transmigration into tissues. Pro-inflammatory cytokines such as IL-1, TNF and IFNγ induce CD54 expression on endothelial cells and fibroblasts. The YN1/1.7.4 antibody has been shown to neutralize CD54 in vivo and inhibit CXCL1, IFNγ, and IL-17 production and neutrophil infiltration into inflammatory lesions.

Isotype

Rat IgG2b

Recomended Isotype Control(s) InVivoMAb rat IgG2b isotype control, anti-keyhole limpet hemocyanin
Recommended InVivoPure Dilution Buffer InVivoPure pH 7.0 Dilution Buffer
Immunogen

NS-1 mouse myeloma cells

Reported Applications
  • in vivo ICAM-1 neutralization
  • Immunohistochemistry (frozen)
  • ELISA
Endotoxin
  • <2EU/mg (<0.002EU/μg)
  • Determined by LAL gel clotting assay
Purity
  • >95%
  • Determined by SDS-PAGE
Formulation
  • PBS, pH 7.0
  • Contains no stabilizers or preservatives
Sterility

0.2 μM filtered

Production

Purified from tissue culture supernatant in an animal free facility

Purification

Protein G

Storage

Undiluted at 4°C in the dark