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InVivoMAb anti-mouse CD45RB
Clone Catalog # Category
MB23G2 (HB220) BE0019 InVivoMab Antibodies
$300 - 3080

The HB220 antibody reacts with mouse CD45RB a 220 kDa transmembrane protein tyrosine phosphatase expressed on peripheral B cells naëâve T cells thymocytes and weakly on macrophages and dendritic cells. CD45RB is one of 5 isoforms of CD45 which result from alternative splicing of exons 4-6. CD45RB results from exon 5 splicing. CD45RB is critical for intracellular signaling in T cells in response to antigen stimulation as indicated by the severe immunodeficiency observed in CD45-deficient mice. As T cells progress from naëâve to memory cells and become activated CD45RB expression is downregulated. Additionally the intensity of CD45RB expression can be used to differentiate between functionally distinct CD4+ T cell subsets which secrete differing cytokine profiles. The primary ligands for CD45 include galectin-1 CD2 CD3 CD4 and Thy-1.

Isotype

Rat IgG2a

Recomended Isotype Control(s) InVivoMAb mouse IgG1 isotype control, unknown specificity
Recommended InVivoPure Dilution Buffer InVivoPure pH 7.0 Dilution Buffer
Immunogen

Anti-immunoglobulin activated mouse B cells

Reported Applications
  • in vivo anti-CD45RB_mediated tolerance induction
  • in vivo pre-mNK cell depletion
Endotoxin
  • <2EU/mg (<0.002EU/μg)
  • Determined by LAL gel clotting assay
Purity
  • >95%
  • Determined by SDS-PAGE
Formulation
  • PBS, pH 7.0
  • Contains no stabilizers or preservatives
Sterility

0.2 μM filtered

Production

Purified from tissue culture supernatant in an animal free facility

Purification

Protein G

Storage

Undiluted at 4°C in the dark