InVivoMAb anti-mouse CD3ε F(ab')2 fragment
Clone Catalog # Category
145-2C11 f(ab')2 Fragments BE0001-1FAB InVivoMab Antibodies
$500 - 3630

The anti-mouse CD3 F(ab')2 fragment is the antigen binding F(ab')2 fragments of the 145-2C11 monoclonal antibody only. The majority of the Fc fragment has been removed via pepsin digestion. The 145-2C11 antibody reacts with mouse CD3ε, a 20 kDa transmembrane cell-surface protein that belongs to the immunoglobulin superfamily. CD3ε is one of five polypeptide chains that combine to form the TCR complex. CD3ε is expressed on T lymphocytes, NK-T cells, and to varying degrees on developing thymocytes. CD3 plays roles in TCR signaling, T lymphocyte activation, and antigen recognition. The 145-2C11 antibody is commonly used to stimulate T cells in vitro however, when used in vivo T cell depletion as well as a marked increase in the release of cytokines such as IL-2 has been reported. To avoid these complications F(ab')2 fragments of the 145-2C11 antibody are commonly used. in vivo administration of 145-2C11 F(ab')2 fragments has been reported to deplete T cells without causing T cell activation or inducing an immune response.


Armenian Hamster IgG1

Recomended Isotype Control(s) InVivoMAb hamster IgG f(ab')2 fragments
Recommended InVivoPure Dilution Buffer InVivoPure pH 7.0 Dilution Buffer

Mouse BM10-37 cytotoxic T cells

Reported Applications

in vivo T cell depletion

  • <2EU/mg (<0.002EU/μg)
  • Determined by LAL gel clotting assay
  • >95%
  • Determined by SDS-PAGE
  • PBS, pH 7.0
  • Contains no stabilizers or preservatives

0.2 μM filtered


Pepsin Digest


Protein A


Undiluted at 4°C in the dark