InVivoMAb anti-mouse CD3ε F(ab')2 fragment

Clone Catalog # Category
145-2C11 f(ab')2 Fragments BE0001-1FAB InVivoMab Antibodies
$500 - $3825
SDS Sheet

About InVivoMAb anti-mouse CD3ε F(ab')2 fragment

The anti-mouse CD3 F(ab')2 fragment is the antigen binding F(ab')2 fragments of the 145-2C11 monoclonal antibody only. The majority of the Fc fragment has been removed via pepsin digestion. The 145-2C11 antibody F(ab')2 fragment reacts with mouse CD3ε, a 20 kDa transmembrane cell-surface protein that belongs to the immunoglobulin superfamily. CD3ε is one of five polypeptide chains that combine to form the TCR complex. CD3ε is expressed on T lymphocytes, NK-T cells, and to varying degrees on developing thymocytes. CD3 plays roles in TCR signaling, T lymphocyte activation, and antigen recognition. The complete 145-2C11 antibody is commonly used to stimulate T cells in vitro however, when used in vivo activation of resting T cells results in cytokine release and toxicity caused by Ab-mediated cross-linking of T cells and Fcγ receptor-bearing cells. To avoid these complications non-Fc receptor binding F(ab’)2 fragments of the 145-2C11 antibody are commonly used. This non-FcR-binding anti-CD3 induces apoptosis of Ag-activated T cells in vivo by allowing durable expression of the TCR and sustained signaling. Foxp3+ Tregs have been shown to be resistant to CD3 antibody-mediated depletion.

InVivoMAb anti-mouse CD3ε F(ab')2 fragment Specifications


Armenian Hamster IgG1

Recommended Isotype Control(s) InVivoMAb hamster IgG f(ab')2 fragments(BE0091-FAB)
Recommended InVivoPure Dilution Buffer InVivoPure pH 7.0 Dilution Buffer(IP0070)

Mouse BM10-37 cytotoxic T cells

Reported Applications

in vivo T cell depletion

  • <2EU/mg (<0.002EU/μg)
  • Determined by LAL gel clotting assay
  • >95%
  • Determined by SDS-PAGE
  • PBS, pH 7.0
  • Contains no stabilizers or preservatives

0.2 μM filtered


Pepsin Digest


Protein A


The antibody solution should be stored undiluted at 4°C, and protected from prolonged exposure to light. Do not freeze.



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