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InVivoMAb anti-mouse CD3ε F(ab')2 fragment
Clone Catalog # Category
145-2C11 f(ab')2 Fragments BE0001-1FAB InVivoMab Antibodies
$500 - 3630

The anti-mouse CD3 F(ab')2 fragment is the antigen binding F(ab')2 fragments of the 145-2C11 monoclonal antibody only. The majority of the Fc fragment has been removed via pepsin digestion. The 145-2C11 antibody reacts with mouse CD3ε, a 20 kDa transmembrane cell-surface protein that belongs to the immunoglobulin superfamily. CD3ε is one of five polypeptide chains that combine to form the TCR complex. CD3ε is expressed on T lymphocytes, NK-T cells, and to varying degrees on developing thymocytes. CD3 plays roles in TCR signaling, T lymphocyte activation, and antigen recognition. The 145-2C11 antibody is commonly used to stimulate T cells in vitro however, when used in vivo T cell depletion as well as a marked increase in the release of cytokines such as IL-2 has been reported. To avoid these complications F(ab')2 fragments of the 145-2C11 antibody are commonly used. in vivo administration of 145-2C11 F(ab')2 fragments has been reported to deplete T cells without causing T cell activation or inducing an immune response.

Isotype

Armenian Hamster IgG1

Recomended Isotype Control(s) InVivoMAb hamster IgG f(ab')2 fragments
Recommended InVivoPure Dilution Buffer InVivoPure pH 7.0 Dilution Buffer
Immunogen

Mouse BM10-37 cytotoxic T cells

Reported Applications

in vivo T cell depletion

Endotoxin
  • <2EU/mg (<0.002EU/μg)
  • Determined by LAL gel clotting assay
Purity
  • >95%
  • Determined by SDS-PAGE
Formulation
  • PBS, pH 7.0
  • Contains no stabilizers or preservatives
Sterility

0.2 μM filtered

Production

Pepsin Digest

Purification

Protein A

Storage

Undiluted at 4°C in the dark