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InVivoMAb anti-mouse CD1d (CD1.1)
Clone Catalog # Category
20H2 (HB323) BE0179 InVivoMab Antibodies
$300 - 3080

The 20H2 monoclonal antibody reacts with mouse CD1d also known as CD1.1. CD1d is a 48 kDa type I membrane glycoprotein and a member of the CD1 family of glycoproteins. CD1d is a non-classical MHC protein with structural homology to class I MHC molecules. CD1d is expressed on the surface of various antigen-presenting cells and is involved in the presentation of non-peptide glycolipid antigens to to CD1d-restricted T cells. CD1d-presented glycolipid antigens activate invariant natural killer T (iNKT) cells, through the interaction with the T-cell receptor present on iNKT cell membranes. When activated, iNKT cells rapidly produce Th1 and Th2 cytokines.

Isotype

Rat IgG1

Recomended Isotype Control(s) InVivoMAb rat IgG1 Isotype control, anti-trinitrophenol
InVivoMAb rat IgG1 isotype control, anti-horseradish peroxidase
Recommended InVivoPure Dilution Buffer InVivoPure pH 7.0 Dilution Buffer
Immunogen

CD1.1-transfected rat cell line RBL-CD1.1 followed by CD1.1-transfected human cell line CR1-CD1.1

Reported Applications
  • iNKT cell neutralization
  • in vivo CD1d blockade
  • Flow cytometry
Endotoxin
  • <2EU/mg (<0.002EU/μg)
  • Determined by LAL gel clotting assay
Purity
  • >95%
  • Determined by SDS-PAGE
Formulation
  • PBS, pH 7.0
  • Contains no stabilizers or preservatives
Sterility

0.2 μM filtered

Production

Purified from tissue culture supernatant in an animal free facility

Purification

Protein G

Storage

Undiluted at 4°C in the dark