InVivoPlus anti-mouse CD25 (IL-2Rα)

Clone Catalog # Category
PC-61.5.3 BP0012 InVivoPlus Antibodies
$455 - $4000
SDS Sheet

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About InVivoPlus anti-mouse CD25 (IL-2Rα)

The PC-61.5.3 monoclonal antibody reacts with mouse IL-2Rα also known as CD25, Ly-43, p55, or Tac. IL-2Rα is the 55 kDa ligand-binding subunit of the interleukin 2 receptor alpha chain. IL-2Rα is expressed on activated mature T and B lymphocytes, thymocyte subsets, pre-B cells, and T regulatory cells. IL-2Rα has been shown to play roles in lymphocyte differentiation, activation, and proliferation. Alone, the IL-2Rα binds IL-2 with relatively low affinity however, when IL-2Rα associates with IL-2Rβ (CD122) and the common gamma chain (CD132) the complex binds IL-2 with high affinity. The PC-61.5.3 antibody has been shown to inhibit the binding of IL-2 to both the low and high affinity IL-2 receptor forms. Additionally, the PC-61.5.3 antibody is commonly used to deplete CD4+FoxP3+ T regulatory cells in vivo.

InVivoPlus anti-mouse CD25 (IL-2Rα) Specifications

Isotype

Rat IgG1, λ

Recommended Isotype Control(s) InVivoPlus rat IgG1 isotype control, anti-horseradish peroxidase(BP0088)
InVivoPlus rat IgG1 isotype control, anti-trinitrophenol(BP0290)
Recommended InVivoPure Dilution Buffer InVivoPure pH 7.0 Dilution Buffer(IP0070)
Immunogen

IL-2-dependent cytolytic mouse T cell clone B6.1

Reported Applications
  • in vivo regulatory T cell depletion
  • Flow cytometry
Endotoxin
  • <1EU/mg (<0.001EU/μg)
  • Determined by LAL gel clotting assay
Purity
  • >95%
  • Determined by SDS-PAGE
Aggregation
  • <5%
  • Determined by DLS
Formulation
  • PBS, pH 7.0
  • Contains no stabilizers or preservatives
Sterility

0.2 μM filtered

Production

Purified from tissue culture supernatant in an animal free facility

Purification

Protein A High Salt

Storage

The antibody solution should be stored undiluted at 4°C, and protected from prolonged exposure to light. Do not freeze.

Murine Pathogen Test Results
  • Mouse Norovirus: Negative
  • Mouse Parvovirus: Negative
  • Mouse Minute Virus: Negative
  • Mouse Hepatitis Virus: Negative
  • Reovirus Screen: Negative
  • Lymphocytic Choriomeningitis virus: Negative
  • Lactate Dehydrogenase-Elevating Virus: Negative
  • Mouse Rotavirus: Negative
  • Theiler's Murine Encephalomyelitis: Negative
  • Ectromelia/Mousepox Virus: Negative
  • Hantavirus: Negative
  • Polyoma Virus: Negative
  • Mouse Adenovirus: Negative
  • Sendai Virus: Negative
  • Mycoplasma Pulmonis: Negative
  • Pneumonia Virus of Mice: Negative
  • Mouse Cytomegalovirus: Negative
  • K Virus: Negative
RRID

AB_1107619

The InVivoPlus Difference

Our InVivoPlus™ antibodies feature all the great qualities of our InVivoMab™ antibodies.  The InVivoPlus™ versions of our products are structurally and functionally identical to the InVivoMab™ versions with the added benefit of additional QC measures. InVivoPlus™ antibodies are screened for murine pathogens using ultrasensitive qPCR, screened for protein aggregation via dynamic light scattering, feature advanced binding validation via flow cytometry, ELISA, and/or Western blot, and are guaranteed to contain less than 1 endotoxin unit per milligram. Our InVivoPlus™ line of antibodies are designed to exceed the strict demands and rigorous standards required for in vivo work at any research organization.