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InVivoMAb anti-mouse VISTA

Clone Catalog # Category
13F3 BE0310 InVivoMab Antibodies
$95 - $3080
SDS Sheet

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About InVivoMAb anti-mouse VISTA

The 13F3 monoclonal antibody reacts with mouse V-domain Ig suppressor of T cell activation (VISTA) also known as PD-1H and B7-H5. VISTA is a 309 aa type I transmembrane glycoprotein and a member of the Ig superfamily. VISTA is expressed on naïve and activated T cells, NK cells, macrophages, dendritic cells, and neutrophils. VISTA functions as a negative immune-checkpoint protein that suppresses T cell cytokine production and proliferation. VISTA is overexpressed by tumor-infiltrating lymphocytes, such as myeloid cells and regulatory T cells. Blockade of VISTA with the 13F3 antibody results in delayed tumor growth in mouse models of melanoma.

InVivoMAb anti-mouse VISTA Specifications

Isotype

Armenian Hamster IgG

Recomended Isotype Control(s) InVivoMAb polyclonal Armenian hamster IgG(BE0091)
Recommended InVivoPure Dilution Buffer InVivoPure pH 7.0 Dilution Buffer(IP0070)
Immunogen

EL4 cells overexpressing mouse VISTA-RFP and then boosted with VISTA-Ig fusion protein

Reported Applications
  • in vivo blocking of VISTA signaling
  • In vitro blocking of VISTA signaling
  • Flow cytometry
Formulation
  • PBS, pH 7.0
  • Contains no stabilizers or preservatives
Endotoxin
  • <2EU/mg (<0.002EU/μg)
  • Determined by LAL gel clotting assay
Purity
  • >95%
  • Determined by SDS-PAGE
Sterility

0.2 μM filtered

Production

Purified from tissue culture supernatant in an animal free facility

Purification

Protein A

Storage

Undiluted at 4°C in the dark