• SHOP
  • QUOTE
InVivoMab anti-mouse IL-3
Clone Catalog # Category
MP2-8F8 BE0282 InVivoMab Antibodies
$300 - 3080

The MP2-8F8 monoclonal antibody reacts with mouse IL-3, a pleiotropic cytokine produced primarily by activated T cells but also by activated mast cells, keratinocytes and neurons/astrocytes. IL-3 is highly species-specific, mature human and mouse IL-3 share only 29% sequence identity and human IL-3 does not show activity on mouse cells. IL-3 can stimulate the proliferation and differentiation of megakaryocytes, neutrophils, and macrophages from bone marrow cultures. Additionally, IL-3 also affects the functional activity of mature mast cells, basophils, eosinophils and macrophages. The MP2-8F8 antibody has been reported to neutralize the bioactivity of natural or recombinant IL-3 as well as stimulate basophil proliferation when administered as a complex with IL-3.

Isotype

Rat IgG1

Recomended Isotype Control(s) InVivoMAb rat IgG1 isotype control, anti-horseradish peroxidase
InVivoMAb rat IgG1 Isotype control, anti-trinitrophenol
Recommended InVivoPure Dilution Buffer InVivoPure pH 7.0 Dilution Buffer
Immunogen

COS-expressed, recombinant mouse IL-3

Reported Applications
  • in vivo IL-3 neutralization
  • in vitro IL-3 neutralization
  • in vivo IL-3 receptor stimulation (as a complex with IL-3)
  • ELISA
  • Flow cytometry
Endotoxin
  • <2EU/mg (<0.002EU/μg)
  • Determined by LAL gel clotting assay
Purity
  • >95%
  • Determined by SDS-PAGE
Formulation
  • PBS, pH 7.0
  • Contains no stabilizers or preservatives
Sterility

0.2 μM filtered

Production

Purified from tissue culture supernatant in an animal free facility

Purification

Protein G

Storage

Undiluted at 4°C in the dark