• SHOP
  • QUOTE
InVivoMAb anti-mouse CLEC9A (CD370)
Clone Catalog # Category
7H11 BE0305 InVivoMab Antibodies
$95 - $3080

The 7H11 monoclonal antibody reacts with mouse CLEC9A (C-type lectin domain family member 9A). CLEC9A, also known as DNGR1 (dendritic cell natural killer lectin group receptor 1) and CD370, is a type II transmembrane glycoprotein with a single extracellular C-type lectin domain. DNGR-1 is restricted in its expression, being found only on CD8α+, CD103+, CD11b subsets of DCs and plasmacytoid DCs. CLEC9A reportedly functions as an endocytic receptor for necrotic cells. It can mediate the cross-presentation of dead-cell associated antigens by dendritic cells in a Syk-dependent manner. It has been shown that targeting antigen to DNGR-1 on DC’s via coupling antigen to the 7H11 antibody can result in activation of antigen specific CD8+ T cell responses in vivo.

Isotype

Rat IgG1, κ

Recomended Isotype Control(s) InVivoMAb rat IgG1 isotype control, anti-horseradish peroxidase
InVivoMAb rat IgG1 Isotype control, anti-trinitrophenol
Recommended InVivoPure Dilution Buffer InVivoPure pH 7.0 Dilution Buffer
Immunogen

RBL-2H3 cells expressing mouse CLEC9A fused to an HA epitope

Reported Applications

in vivo Ag targeting to CLEC9A+ DCs

Western blot

ELISA

Immunoprecipitation

Immunofluorescence

Flow cytometry

Endotoxin
  • <2EU/mg (<0.002EU/μg)
  • Determined by LAL gel clotting assay
Purity
  • >95%
  • Determined by SDS-PAGE
Formulation
  • PBS, pH 7.0
  • Contains no stabilizers or preservatives
Sterility

0.2 μM filtered

Production

Purified from tissue culture supernatant in an animal free facility

Purification

Protein G

Storage

Undiluted at 4°C in the dark

RRID

AB_2721034