InVivoMAb anti-mouse CD172a (SIRPα)

Clone Catalog # Category
P84 BE0322 InVivoMab Antibodies
$95 - $3250
SDS Sheet

About InVivoMAb anti-mouse CD172a (SIRPα)

The P84 monoclonal antibody reacts with Signal-Regulatory Protein α (SIRPα), also known as CD172a. SIRPα is a type I transmembrane glycoprotein expressed on monocytes, macrophages, and dendritic cells. Neurons and other tissues of the central nervous system have also been shown to express SIRPα. Its ligand, CD47 is expressed by a wide variety of cells. SIRPα and CD47 regulate dendritic cell-mediated T cell activation, neutrophil migration, and phagocytosis. SIRPα diffuses laterally on the macrophage membrane and accumulates at a phagocytic synapse to bind CD47 which inhibits phagocytosis by macrophages. Anti-SIRPα antibodies that block the interaction of SIRPα with CD47 have been shown to suppress tumor formation in mice. The P84 antibody has been shown to have neutralizing activity in vivo and in vitro.

InVivoMAb anti-mouse CD172a (SIRPα) Specifications

Isotype

Rat IgG1, κ

Recommended Isotype Control(s) InVivoMAb rat IgG1 isotype control, anti-horseradish peroxidase(BE0088)
Recommended InVivoPure Dilution Buffer InVivoPure pH 7.0 Dilution Buffer(IP0070)
Immunogen

Mouse brain membrane protein

Reported Applications
  • in vivo SIRPα blocking
  • In vitro SIRPα blocking
  • Western blot
  • Immunoprecipitation
  • Flow cytometry
Formulation
  • PBS, pH 7.0
  • Contains no stabilizers or preservatives
Endotoxin
  • <2EU/mg (<0.002EU/μg)
  • Determined by LAL gel clotting assay
Purity
  • >95%
  • Determined by SDS-PAGE
Sterility

0.2 μM filtered

Production

Purified from tissue culture supernatant in an animal free facility

Purification

Protein A

Storage

Undiluted at 4°C in the dark

Molecular Weight

150 kDa

References provided by Bioz:

Disclaimer: These articles are provided by Bioz, an automated third-party article search engine. Given Bioz’s automation, these articles may not reflect the reported applications listed for this product. For an extensive up-to-date article list directly tailored to the reported applications listed for this product please see the Bio X Cell Application References by clicking the green button above.


Powered by Bioz

We're Heading to AACR

Come visit us at Booth 1842! See you in Atlanta!