InVivoMAb anti-mouse BTLA (CD272)

Clone Catalog # Category
6F7 BE0304 InVivoMab Antibodies
$95 - $3080
SDS Sheet

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About InVivoMAb anti-mouse BTLA (CD272)

The 6F7 monoclonal antibody reacts with mouse B- and T-lymphocyte attenuator (BTLA) also known as CD272. BTLA is an Ig superfamily member which is expressed on B cells, T cells, macrophages, dendritic cells, NK cells, and NKT cells. Like PD-1 and CTLA-4, BTLA interacts with a B7 homolog, B7-H4.  However, unlike PD-1 and CTLA-4, BTLA displays T cell inhibition via interaction with tumor necrosis family receptors, not just the B7 family of cell surface receptors. BTLA is a ligand for herpes virus entry mediator (HVEM). BTLA-HVEM complexes have been shown to negatively regulate T cell immune responses. There are conflicting reports concerning the activity of the 6F7 antibody. In some cases, the antibody is reported to deplete BTLA+ B and CD4 T cells while in others the antibody is reported as having agonistic activity. See the references for details.

InVivoMAb anti-mouse BTLA (CD272) Specifications

Isotype

Mouse IgG1, κ

Recommended Isotype Control(s) InVivoMAb mouse IgG1 isotype control, unknown specificity(BE0083)
Recommended InVivoPure Dilution Buffer InVivoPure pH 7.0 Dilution Buffer(IP0070)
Immunogen

Ig domain of C57BL/6 mouse BTLA

Reported Applications
  • in vivo BTLA+ B cell and CD4 T cell depletion*
  • Flow cytometry

*see description for details

Endotoxin

<2EU/mg (<0.002EU/μg)

Determined by LAL gel clotting assay

Purity

>95%

Determined by SDS-PAGE

Formulation

PBS, pH 7.0

Contains no stabilizers or preservatives

Sterility

0.2 μM filtered

Production

Purified from tissue culture supernatant in an animal free facility

Purification

Protein G

Storage

The antibody solution should be stored undiluted at 4°C, and protected from prolonged exposure to light. Do not freeze.

RRID

AB_2721033