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InVivoMAb anti-human CD54 (ICAM-1)
Clone Catalog # Category
R6-5-D6 BE0020-2 InVivoMab Antibodies
$300 - 3080

The R6-5-D6 monoclonal antibody reacts with human CD54 also known as Intercellular Adhesion Molecule-1 (ICAM-1), is a single-chain transmembrane glycoprotein with a polypeptide core of 55 kDa and a member of the Ig superfamily. CD54 is typically expressed on non-hematopoietic cells such as endothelial cells, thymic epithelial cells, and fibroblasts but can also be expressed on macrophages, T-lymphoblasts, germinal center B cells and dendritic cells. CD54 is a ligand for LFA-1, a receptor found on leukocytes. When activated, leukocytes bind to endothelial cells via CD54/LFA-1 and then transmigrate into tissues. Pro-inflammatory cytokines such as IL-1, TNF and IFNγ induce CD54 expression on endothelial cells and fibroblasts. The R6-5-D6 antibody has been reported to inhibit the interaction of CD54 with leukocytes, thereby decreasing leukocyte adhesion to the vascular endothelium and inflammatory tissue injury.

Isotype

Mouse IgG2a

Recomended Isotype Control(s) InVivoMAb mouse IgG2a isotype control, unknown specificity
Recommended InVivoPure Dilution Buffer InVivoPure pH 7.0 Dilution Buffer
Immunogen

EBV transformed lymphoblast cell line

Reported Applications
  • in vitro T cell stimulation/activation
  • Immunofluorescence
Endotoxin
  • <2EU/mg (<0.002EU/μg)
  • Determined by LAL gel clotting assay
Purity
  • >95%
  • Determined by SDS-PAGE
Formulation
  • PBS, pH 7.0
  • Contains no stabilizers or preservatives
Sterility

0.2 μM filtered

Production

Purified from tissue culture supernatant in an animal free facility

Purification

Protein G

Storage

Undiluted at 4°C in the dark