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InVivoMAb anti-mouse MHC Class I (H-2Dk, H-2Kd, H-2Kf)
Clone Catalog # Category
15-5-5S (HB24) BE0229 InVivoMab Antibodies
$300 - 3080

The 15-5-5S monoclonal antibody is reported to react with the mouse H-2Dk, H-2Kd, and H-2Kf MHC class I alloantigens. MHC class I antigens are heterodimers consisting of one alpha chain (44 kDa) associated with β2 microglobulin (11.5 kDa). The antigen is expressed by all nucleated cells at varying levels. MHC Class I molecules present endogenously synthesized antigenic peptides to CD8 T cells.

Isotype

Mouse IgG2a

Recomended Isotype Control(s) InVivoMAb mouse IgG2a isotype control, unknown specificity
Recommended InVivoPure Dilution Buffer InVivoPure pH 7.0 Dilution Buffer
Immunogen

C3H mouse spleen cells

Reported Applications
  • Functional assays
  • Flow cytometry
Endotoxin
  • <2EU/mg (<0.002EU/μg)
  • Determined by LAL gel clotting assay
Purity
  • >95%
  • Determined by SDS-PAGE
Formulation
  • PBS, pH 7.0
  • Contains no stabilizers or preservatives
Sterility

0.2 μM filtered

Production

Purified from tissue culture supernatant in an animal free facility

Purification

Protein G

Storage

Undiluted at 4°C in the dark