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InVivoMAb anti-human CD4
Clone Catalog # Category
RPA-T4 BE0288 InVivoMab Antibodies
$95 - $3080

The RPA-T4 monoclonal antibody reacts with the human CD4. The CD4 antigen is a 55 kDa cell surface type I membrane glycoprotein belonging to the immunoglobulin superfamily. CD4 acts as a co-receptor which in cooperation with the T cell receptor (TCR) interacts with class II MHC molecules displayed by antigen presenting cells (APC). CD4 is expressed by most thymocytes and helper T cells, a subset of NK-T cells and weakly by dendritic cells and macrophages. CD4 plays an important role in the development of T cells and is required for mature T cells to function optimally. The RPA-T4 antibody is reported to bind to the D1 domain of CD4 and does not block the binding of the OKT-4 antibody. Additionally, RPA-T4 has been shown to block the binding of HIV gp120 protein to CD4 and inhibit CD4 T cell activation in vitro.

Isotype

Mouse IgG1, κ

Recomended Isotype Control(s) InVivoMAb mouse IgG1 isotype control, unknown specificity
Recommended InVivoPure Dilution Buffer InVivoPure pH 7.0 Dilution Buffer
Immunogen

Not available or unknown

Reported Applications
  • in vitro CD4 blockade
  • in vitro blocking of CD4+ T cell activation
  • Immunofluorescence
  • Immunohistochemistry (frozen)
  • Flow cytometry
Endotoxin
  • <2EU/mg (<0.002EU/μg)
  • Determined by LAL gel clotting assay
Purity
  • >95%
  • Determined by SDS-PAGE
Formulation
  • PBS, pH 7.0
  • Contains no stabilizers or preservatives
Sterility

0.2 μM filtered

Production

Purified from tissue culture supernatant in an animal free facility

Purification

Protein G

Storage

Undiluted at 4°C in the dark

RRID

AB_2687811