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InVivoMAb anti-human IFN╬│
Clone Catalog # Category
B133.5 BE0235 InVivoMab Antibodies
$300 - 3080

The B133.5 monoclonal antibody reacts with human IFN╬│ (interferon gamma) a 20 kDa soluble pleiotropic cytokine and the sole member of the type II class of interferons. IFN╬│ is primarily produced by activated lymphocytes including T, B, NK cells, and ILCs. IFN╬│ exerts immunoregulatory, anti-proliferative, anti-viral, and proinflammatory activities and plays an important role in activation, growth, and differentiation of T and B lymphocytes, macrophages, NK cells and other non-hematopoietic cell types. Additionally, IFN╬│ induces the production of cytokines, Fc receptor, and adhesion molecules and up-regulates MHC class I and II antigen expression by antigen presenting cells during an immune response. IFN╬│ has also been shown to modulate macrophage effector functions, influence isotype switching and induce the secretion of immunoglobulins by B cells. IFN╬│ signals through the IFN gamma receptor which exists as a heterodimer composed of CD119 (IFN gamma receptor 1) and AF-1 (IFN gamma receptor 2). The IFN╬│ receptor is expressed ubiquitously on almost all cell types with the exception of mature erythrocytes. The B133.5 antibody is a neutralizing antibody.

Isotype

Mouse IgG1

Recomended Isotype Control(s) InVivoMAb mouse IgG1 isotype control, unknown specificity
Recommended InVivoPure Dilution Buffer InVivoPure pH 7.0 Dilution Buffer
Immunogen

Recombinant human IFN╬│

Reported Applications

in vitro IFN╬│ neutralization

Endotoxin
  • <2EU/mg (<0.002EU/╬╝g)
  • Determined by LAL gel clotting assay
Purity
  • >95%
  • Determined by SDS-PAGE
Formulation
  • PBS, pH 7.0
  • Contains no stabilizers or preservatives
Sterility

0.2 ╬╝M filtered

Production

Purified from tissue culture supernatant in an animal free facility

Purification

Protein G

Storage

Undiluted at 4┬░C in the dark