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InVivoMAb anti-mouse F4/80
Clone Catalog # Category
CI:A3-1 BE0206 InVivoMab Antibodies
$300 - 3080

The CI:A3-1 monoclonal antibody reacts with mouse F4/80 also known as EMR1. F4/80 is a 160 kDa membrane glycoprotein belonging to the EGF-TM7 family. F4/80 is expressed on most tissue macrophages including peritoneal, lung, gut, thymus and splenic red pulp macrophages, as well as Kuffer cells, Langerhans cells, bone marrow stromal cells, and a subset of dendritic cells. F4/80's cognate ligand has not been identified however studies in mice have shown that F4/80 is important for cytokine production and induction regulatory T cells.

Isotype

Rat IgG2b

Recomended Isotype Control(s) InVivoMAb rat IgG2b isotype control, anti-keyhole limpet hemocyanin
Recommended InVivoPure Dilution Buffer InVivoPure pH 7.0 Dilution Buffer
Immunogen

C57BL/6 mouse thioglycollate stimulated peritoneal macrophages

Reported Applications
  • in vivo Monocyte/Macrophage depletion
  • Functional assays
  • Immunohistochemistry (paraffin)
  • Immunohistochemistry (frozen)
  • Flow cytometry
Endotoxin
  • <2EU/mg (<0.002EU/μg)
  • Determined by LAL gel clotting assay
Purity
  • >95%
  • Determined by SDS-PAGE
Formulation
  • PBS, pH 7.0
  • Contains no stabilizers or preservatives
Sterility

0.2 μM filtered

Production

Purified from tissue culture supernatant in an animal free facility

Purification

Protein G

Storage

Undiluted at 4°C in the dark