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Posted on: September 3, 2019
InVivoMAb anti-mouse IL-27 p28
The MM27.7B1 monoclonal antibody reacts with the p28 subunit of mouse IL-27, a member of the IL-6/IL-12 cytokine family. IL-27 is a heterodimeric cytokine composed the EBI3 (Epstein-Barr virus induced gene 3) protein and the p28 subunit, also known as IL-30. Myeloid cells including macrophages, inflammatory monocytes, microglia, and dendritic cells are the dominant cellular sources of IL-27. These cells secrete IL-27 in response to TLR ligands and inflammatory cytokines. IL-27 p28 signals through IL-27 receptors (IL-27R) which are highly expressed by NK cells and activated T cells and to a lesser extent on B cells, naïve T cells, and others. IL-27 signaling has been shown to have both pro-inflammatory and anti-inflammatory effects. Signaling through IL-27R promotes Th1 polarization and IFNγ production but also suppresses the differentiation and proliferation of Th2 and Th17 cells and induces the expression of IL-10. The MM27.7B1 clone has been shown to neutralize the bioactivity of IL-27 in vivo and in vitro.
This Antibody Is Useful For:
- in vivo IL-27 p28 neutralization
- in vitro IL-27 p28 neutralization
- Flow cytometry
As always Bio X Cell antibodies are specifically formulated for in vivo use and feature:
- 95% purity
- Ultra-low endotoxin levels
- Preservative, stabilizer, and carrier protein-free
Read how other researchers have used the anti-mouse IL-27 p28 antibody.
Recommended Control and Buffer
InVivoMAb mouse IgG2a isotype control, unknown specificity
The C1.18.4 monoclonal antibody is ideal for use as a non-reactive isotype-matched control for mouse IgG2a antibodies in most in vivo and in vitro applications.
InVivoPure pH 7.0 Dilution Buffer
InVivoPure™ dilution buffers are specifically formulated and tested to satisfy the stringent requirements for in vivo applications. They are extremely low in endotoxin, have been screened for murine pathogens, tested in animal models for toxicity and are formulated with respect to buffer composition and pH to satisfy the requirements of Bio X Cell’s antibodies.