Bio X Cell offers custom fermentation and purification services for the production of monoclonal antibodies in cell culture.
Bio X Cell employs state of the art fermentation technologies; including stirred tank fermenters and wavebag systems; our production capacity ranges from 5 to 500L.
Custom Production of Antibodies
Bio X Cell offers custom production services of antibodies produced from hybridomas supplied by our clients. These hybridomas are either 1.) developed in the clients laboratory or 2.) available in the public domain. In this case we would sign a Material Transfer Agreement if requested. Many scientifically important antibodies have been supplied on a custom production basis to our clients. These antibodies include:
This service includes fermentation of the hybridoma in cell culture, harvest of the cell culture supernatant by centrifugation and concentration by 50kD ultrafiltration. Purification is performed by Protein A or G affinity chromatography. The purified antibody is dialyzed into PBS and filter sterilized. All our of our custom products will be delivered with an endotoxin level <2EU/mg. If lower endotoxin levels are required contact customer service. For an extra charge we offer freezing of the cell line and MAP testing.